The study is aimed at isolation and characterization of microsatellites from the wild silkmoth Antheraea mylitta. Microsatellites isolation was preformed from a small – insert genomic library followed by colony screening using microsatellite specific primers containing different nucleotide arrays.
In the present study six microsatellite specific primers of the Di- and Trinucleotied repeat types were used as primers in PCR screening for microsatellite containing clones. Genomic DNA was digested with Say3A. size selected from 500 - 1000 bp and dephorylated genoinic DNA was cloned into Bamh I digested pBluescript (SK) plasmid and transformed into INV aF competent cells.
Totally 65 white colonies were screened for the presence of microsatellite with microsatellite specific primers. Multiple PCR products obtained using a combination of Universial (M13 F/R) and repeat primers of sizes above 200 bp were selected and sequenced.
Totally 10 clones found positive for the present of microsatellites. Out of 10 clones, only 5 were found to be positive for microsatellites by sequencing. The type of micro satellite repeat found was Di- and Trinucleotide repeats. The availability of micro satellite markers can be expected to enhance the power and resolution of genomic anolysis in the wild silk moth Antheraea mylitra.
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