ABSTRACT

As part of a study to map yield enhancing QTLs from O.nivara  and introgress them into varieties, hybrids were obtained using Swarna (O.sativa) as female and two O. nivara accessions as male parents. IRGC 81832 (SL -11) from Bihar and IRGC 81842 (SL -12) from Uttar Pradesh were selected based on their molecular diversity and vigour.  116 microsatellite markers were screened to identify polymorphic loci between the parents.  The number of SSR primers screened per chromosome ranged form 6 to 14 and those polymorphic ranged from 4 to 10.  Overall 85 (73%) markers showed polymorphism.

10 RAPD primers were screened and 3 showed polymorphism. 10 ISSR, primers were screened and two showed polymorphism. 44 of the 48  F1 plants were identified as true hybrids using  molecular markers which were specific to the male parent (O. nivara). These were 4 and 3 bands amplified by ISSR primers UBC 811 and UBC 834, respectively and one band each amplified by the three RAPD primers OPA 7, OPA 10 and OPB2. These can be used as diagnostic markers for hybrid confirmation. The polymorphic markers between O. sativa and O. nivara will be useful in studies on QTL mapping and assessing gene flow from wild rice to cultivated and vice versa.