ABSTRACT

The detection of genetically modified foodstuffs is becoming both a food sales and legal necessity. Hence, analytical methods are necessary to show the presence of GMO in raw materials and processed foods. This study reports a PCR based method for the detection of genetically modified cotton and rice. Detection of transgenes, LOD (Limit of Detection), and LOQ (Limit of Quantitation) of the same transgenes are the two objectives undertaken in the present study.

DNA extraction methods were standardized and the PCR conditions were optimized for all tarnsgenes in Bt cotton and Bt rice. In this present study, the LOD for all target genes was further improved to 0.04% by accurate mixing of Bt and non-Bt cotton/rice seed powder followed by genomic DNA extraction. All the results were confirmed on 5% PAGE using ABI-Prism and analysis was done by using “Genotyper 2.5” software and the LOQ was confirmed to 0.05%. Sampling was done to the same thresholds in large samples.